Analysis of ciliary development in Scgb3a1(–/–) and Scgb3a2(–/–) mouse lungs. (A) Percentage of ciliated cells in large airways of Scgb3a1(–/–) (3a1-KO), Scgb3a2(–/–) (3a2-KO), and their WT littermates mice. Mice used for this analysis were those 10 times backcrossed to C57BL/6N mice. Statistical difference between WT and 3a2-KO was obtained using one-way ANOVA. (B) Representative β-tubulin immunostaining using lungs of Scgb3a1(–/–) (KO), Scgb3a2(–/–) (KO), and their respective WT littermates. Airways shown include both large and small airways. Counterstained with light green. Scale bar: 50 µm for all panels. Abbreviation: WT, wild-type; KO, knockout.