Figure 9.

PC2 expression in islets of T2D donors. Photomicrographs A to C show islets from T2D donor #6139 immunostained for insulin (red) and PC2 (green). Note that beta cells in islets with abnormal morphology, such as those shown in Fig. 7A, maintain PC2 expression. Insert in Fig. 7A is a high magnification image of the area indicated with an arrow illustrating double-label IN⁺PC2⁺ cells and IN⁻PC2⁺ cells. Bar for Fig. 9A to C: 25 µm. (D and E) Graphs indicate the total fluorescence (in a.u.) of islets from donors #6139 and #6127. Although there is variation in the values of enzyme levels, this disparity is less pronounced than that found in islets stained for PC1/3. Abbreviations: PC2, proprotein convertase 2; IN⁺, insulin+; T2D, type 2 diabetes; PC1/3, proprotein convertase 1/3; a.u., arbitrary unit.