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. 2019 May 29;8(1):796–807. doi: 10.1080/22221751.2019.1621670

Figure 2.

Figure 2.

The cytotoxic effect of TcdB and TcdB-F on VERO cells. VERO cells were treated with the supernatant of 72-hour-old cultures of C. difficile strain 2149 (RT 014/020 which produces TcdB), C. difficile strain 1470 (RT 017 which produce TcdB-F), and C. difficile ATCC 700057 (RT 038 which is non-toxigenic) and incubated at 37°C for 24 hours before inspection under a light microscope. TcdB glycosylates Rho, Rac, and Cdc42 targets resulting in arborization of cells while TcdB-F glycosylates Rac and Ras targets resulting in rounding of cells without arborization.