Fig. 3.

Mouse PXR agonists fail to evoke caspase-1 activation or IL-1β release in peritoneal macrophages isolated from PXR^−/− mice. The PXR is expressed in LPS-pulsed peritoneal macrophages (A) and is required for PCN-induced caspase-1 activation [as assessed by detecting the cleaved caspase-1 p20 subunit in the supernatant (B and C)] and subsequent IL-1β release (D), measured by enzyme-linked immunosorbent assay. CL, cell lysate; SN, supernatant. All outcomes were measured after a 6-hour treatment period. N = 3–6; *, denotes P < 0.05 for indicated comparison generated by analysis of variance and Tukey’s post hoc test; Western blots are representative of three separate experiments.