Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 May 28;8(1):773–786. doi: 10.1080/22221751.2019.1618686

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2019 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group, on behalf of Shanghai Shangyixun Cultural Communication Co., Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

Figure 4. — Endophilin-A2-mediated endocytic pathway is critical for EV71 to enter Caco-2 cells (a) Kinetic curve of EV71 entry into Caco-2 cells. Results are shown as a percentage of internalized virus compared with control in which PBS was substituted for proteinase K. (b) The effectiveness of proteinase K (Pse-K) treatment was evaluated by qRT-PCR. Values are normalized to PBS control (Ctrl). (c, d) EV71 (MOI = 10) and EV71 (FY)-Luc infectivity of Caco-2 cells transfected with siRNA targeting endophilin family members (A1, A2, A3, B1, and B2). Values are normalized to non-targeting siRNA (siNT) control. (e) Confocal microscopy localization of endophilin-A2 (green) with EV71 (MOI = 100) (red) after infected by 30 and 90 min. Nuclei were stained with DAPI. Insets show higher magnification of boxed areas. Scale bars, 20 μm. (f) Confocal microscopy localization of endophilin-A2 (green) with dynamin 2 (red) or actin (red) in Caco-2 cells after EV71 (MOI = 100) infected by 60 min. Insets show higher magnification of boxed areas. Nuclei were stained with DAPI. Scale bars, 20 μm. **p < 0.01.