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. 2016 Oct 13;97(10):2668–2676. doi: 10.1099/jgv.0.000590

Fig. 2.

Fig. 2.

Impact of different combinations of BCP mutations on HBV biological properties. The T1753C (simplified as 53), A1762T (62), G1764A (64) and C1766T (66) mutations were introduced to clone 2A of genotype A in various combinations, and tandem EcoRI dimers were transiently transfected to Huh7 cells. HBV 5.4 was defective in genome replication and HBeAg expression. (a) Northern blot analysis of HBV RNAs using glyceraldehyde-3-phosphate dehydrogenase (GAPDH) as a loading control. Southern blot analysis of (b) intracellular replicative DNA and (c) extracellular virion DNA. (d) Western blot analysis of intracellular envelope and core proteins using β-actin as a loading control. (e) Western blot analysis of secreted S protein following polyethylene glycol (PEG) precipitation. (f) HBsAg and HBeAg values averaged from seven transfection experiments, with those from clone 2A set at 100 %. *P<0.05.