Skip to main content
. 2016 Oct 13;97(10):2668–2676. doi: 10.1099/jgv.0.000590

Fig. 5.

Fig. 5.

Overproduction of either the 2.4/2.1 kb RNA diminished 0.7 kb RNA and HBx protein. (a) A 0.7-mer (2.4 kb) or 0.6-mer (2.1 kb) HBV sequence of clone 6.2 or 21.2, with or without a CMV promoter at the 5′ end, was inserted to a modified pUC18 vector. This will ensure overproduction or no production of the 2.4 kb RNA (0.7-mer construct), or overproduction or no production of the 2.1 kb RNA (0.6-mer construct). The 0.7-mer and 0.6-mer constructs were separately transfected to Huh7 cells. (b) Northern blot of HBV RNAs using glyceraldehyde-3-phosphate dehydrogenase (GAPDH) as a loading control. Note that for the 0.7-mer constructs, the 2.4 and 2.1 kb RNAs cannot be resolved into separate bands. (c) Western blot analysis of intracellular envelope and HBx proteins, using β-actin as a loading control. (d) Western blot analysis of extracellular S protein following polyethylene glycol (PEG) precipitation. (e) Secreted HBsAg averaged from seven transfection experiments. Both, for the 0.7-mer and 0.6-mer constructs, the values for 6.2 and 21.2 constructs without a CMV promoter were set at 100 %. *P<0.05.