Design, construction and characterization of RNA micelles. (A) 3WJ motif of pRNA from bacteriophage phi29 DNA packaging motor. (B) Illustration of pRNA-3WJ micelles formation by hydrophobic force. (C) 2D structure of 3WJ/FA/anti-miR21 micelles (underlined sequence is 8nt LNA modified anti-miR21 seed region). (D) Assembly of RNA micelles assayed by 2% agarose gel. (Lane from left to right: 3WJ, 3WJ micelles, 3WJ/anti-miR21, 3WJ/anti-miR21 micelles, 3WJ/FA/anti-miR21, and 3WJ/FA/anti-miR21 micelles). (E) Size distribution of 3WJ/FA/anti-miR21 micelles. (F) ζ potential of 3WJ/FA/anti-miR21 micelles.