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Assembly of circular dsDNA encoded for a single pRNA 3WJ strand surrounded by the ribozyme for cleavage. Representative gel image of the assembly process. (1) Single-stranded linear DNA (ssDNA Lin); (2) single-stranded circular DNA (ssDNA Circ), (3) double-stranded linear DNA (dsDNA Lin); (4) double-stranded circular DNA (dsDNA Circ) + Nick; (5) dsDNA Circ + T4 Ligation to close nick. Ultralow range DNA Ladder (ULR) and 100bp DNA ladder for size comparison.
