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. Author manuscript; available in PMC: 2020 Apr 23.
Published in final edited form as: ACS Nano. 2019 Mar 22;13(4):4603–4612. doi: 10.1021/acsnano.9b00649

Figure 3.

Figure 3.

RCT assessment. Inactive ribozymes lead to concatemerized RNA, whereas cleavage results in short oligomers. RNA products of transcriptions using (1) single-stranded linear DNA (ssDNA Lin); (2) single-stranded circular DNA (ssDNA Circ); (3) double-stranded linear DNA (dsDNA Lin); (4) double-stranded circular DNA (dsDNA Circ) + Nick; (5) dsDNA Circ; (6) dsDNA Lin; (7) dsDNA Circ + Nick; (8) dsDNA Circ. Lanes 3–5 use inactivated ribozyme (RBZ) sequences; lanes 6–8 use active ribozyme sequences. Gel bands were quantified for desired products from lanes 6–8. Additional band details are provided in Supp Figure 1.