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. Author manuscript; available in PMC: 2020 Apr 23.
Published in final edited form as: ACS Nano. 2019 Mar 22;13(4):4603–4612. doi: 10.1021/acsnano.9b00649

Figure 4.

Figure 4.

In situ or one-pot co-transcriptional nanoparticles. (A) Circular (Circ) and linear (Lin) transcription for dsDNA constructs encoding for release of the 3WJ ssRNA oligomers.49 3WJ-a-Lin; (2) 3WJ-a-Circ; (3) 3WJ-b-Lin; (4) 3WJ-b-Circ; (5) 3WJ-c-Lin; (6) 3WJ-c-Circ; (7) 5′/3′Rbz-IN; (8) 3′Rbz-IN; (9) 5′Rbz-IN; (10) 5′Rbz Only; (11) 3′Rbz Only. Gel bands were quantified for desired products from lanes 1–5. Additional band details are provided in Supp Figure 1. (B) Assembly of the 3WJ occurs co-transcriptionally (CoT) by mixing equimolar amounts of dsDNA encoding for the release of 3WJ-a, -b, and -c strands. Rolling circle transcription (RCT)-3WJ is assembled from purified RNA fragments from RCT reaction. Ultralow range DNA Ladder (ULR) for comparison.