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. Author manuscript; available in PMC: 2019 May 30.
Published in final edited form as: Cell Rep. 2018 Aug 28;24(9):2329–2341.e8. doi: 10.1016/j.celrep.2018.07.097

Figure 6. hNeP produce only neutrophils in NSG-SGM3 (NSG-M3) mice in vivo.

Figure 6

(A) Scheme showing the experimental procedure. CD34+ hNeP and CD34−hNeP subsets identified in Figure 5C were sorted from healthy human BM, and adoptively transferred into NSG-M3 recipient mice. Each recipient mouse received 25,000 donor human hNeP progenitor cells. After the transfer, peripheral blood was collected from each recipient via saphenous vein for flow cytometry on Day (D) 5, 7, 14, 28 (D5, D7, D14, D28), respectively. (B) Representative plots show the appearance of monocytes (CD86+ CD66b−), neutrophils (CD86−Siglec 8−CD66b+), eosinophils (Siglec 8+), and lymphocytes (hLy+) in each recipient group at the time points indicated. hLy antibody cocktail contains CD3, CD19, and CD56. N = 10 mice for each group.