Figure 4.
Protein expression levels of the components of GWI hippocampal CICR machinery. Western immunoblots of hippocampal protein from DFP and age-matched control (CTL) rats were stained for A, PKA RII; B, PLCγ1; C, RyR2; D, IP3R1; E, phospho-RyR2; and F, Calstabin2. To adjust for sample loading, the blots were stained with an antibody specific for the 42 kDa β-actin protein. G, Densitometric analyses of the Western blotting revealed no significant changes in proteins (A–E) but identified significant reduction in the levels of Calstabin2 (F) compared with age-matched control (Data expressed as mean optical density ± SEM, *p < .05, t test, n = 6 rats).