Figure 2.
(A) HS-5 cells catalyzed conversion of pD1-B within 24 h. (B) HS-5 cells treated with pD1-B (200 μM) for 24h and then stained with FITC-avidin for 2h. Scale bar is 10 μm. (C) 3D construction of HS-5 cells treated with NBD1P-B (200 μM) for 24 h. (D) 3D construction (see videos S2 and S6) of immunofluorescence staining of HS-5 cells treated with NBD1P-B (200 μM) for 24 h: the cell spheroids stained with antibodies of ECM molecules (i.e., laminin, collagens III and IV, fibronectin) and adhesive molecules (i.e., N-cadherin; integrins α5, β1, and β3). Green indicates the assemblies, red the antibodies, and blue the nuclei. Arrows point to the co-localization of the assemblies and the antibodies.