Table 1.
The sequences pDNA, MT, WT, MTrDNA, and WTrDNA and the melting temperatures (Tm) for MT with pDNA, WT with pDNA, MTrDNA MT, and WTrDNA with WT adjusted for salt concentration in PBS.
| Type of DNA | Sequencea | Tm (°C) |
|---|---|---|
| pDNA | 5’-ACAAAGATCA TTAACC-3’ |
|
| MT | 5’-TTTAAAGACTGGGA GGAGTTGGGGGAGGAG ATTAGGTTAATGATCTT TGT-3’ |
47 |
| WT | 5’-GGTTAAAGGTCTTTG TACTAGGAGGCTGTAG GCATAAATTGGTCTGTT CA-3’ |
23 |
| MT rDNA | 5’-AATCTCCTCCCCCAA CTCCTCCCAGTCTTT-3’ |
77 |
| WT rDNA | 5’-ACAGACCAATTTATG CCTACAGCCTCCTAG-3’ |
76 |
a
Note the mutated sites of the MT are in bold type.