Fig 5. Sdc-1 silencing upregulates IL-23 and DLL4 expression in SUM-149 cells.

48 hours post transfection of SUM-149 with negative control and Sdc-1 siRNA, total RNA was extracted, reverse transcribed into cDNA and relative mRNA expression was quantified by qPCR. mRNA expression of a) IL-23, and b) DLL4 in control and Sdc-1-silenced SUM-149 cells. c) Western blot shows upregulation of DLL4 protein expression in Sdc-1-silenced SUM-149 cells relative to control. Data represent mean ± SEM, n ≥ 3, ** P < 0.01 as determined by Student’s t test.