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. 2019 May 30;15(5):e1007710. doi: 10.1371/journal.ppat.1007710

Fig 2. Requirement of SPI-1 for spread of MVA in human cells.

Fig 2

(A) Effect of addition of the SPI-1gene to MVA. CEF, BS-C-1, HeLa, A549 and MRC-5 cells in 12-well plates were infected with MVA or MVA-SPI-1 at MOI of 0.001, 0.01 and 0.1 and overlaid with methylcellulose. After 48 h, the overlay was removed and cells were stained with antibody to VACV. (B) Effect of SPI-1 gene deletion on v51.2 spread. CEF, BS-C-1, HeLa, A549 and MRC-5 cells in 12-well plates were infected with v51.2 or v51.2ΔSPI at MOI of 0.001, 0.01 and 0.1 and overlaid with methylcellulose. After 48 h, the overlay was removed and cells were stained with antibody to VACV.