| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| C.elegans STRAINS | ||
| wyIs427[Pdat-1::GFP::ELKS-1, Pdat-1::mcherry, Podr-1::RFP] | this study | TV14139 |
| wyIs557[Pdat-1::GFP::ELKS-1, Pdat-1::tdTomato::RAB-3, Podr-1::GFP] | this study | TV15123 |
| thoc-5(wy822); wyIs427[Pdat-1::GFP::ELKS-1, Pdat-1::mcherry, Podr-1::RFP] | this study | TV14937 |
| thoc-5(wy822); wyIs557[Pdat-1::GFP::ELKS-1, Pdat-1::tdTomato::RAB-3, Podr-1::GFP] | this study | TV16068 |
| wyIs690[Pdat-1::CLA-1::GFP, Pdat-1::mcherry, Podr-1::RFP] | this study | TV18497 |
| thoc-5(wy822); wyIs690[Pdat-1::CLA-1::GFP, Pdat-1::mcherry, Podr-1::RFP] | this study | TV18706 |
| wyIs692[Pdat-1::3xnovo2GFP::SYD-2, Pdat-1::mcherry, Podr-1::RFP] | this study | TV18524 |
| thoc-5(wy822); wyIs692[Pdat-1::3xnovo2GFP::SYD-2, Pdat-1::mcherry, Podr-1::RFP] | this study | TV18689 |
| wyIs513[Pdat-1::GFP::SNN-1b, Pdat-1::mcherry, Podr-1::RFP] | this study | TV14859 |
| thoc-5(wy822); wyIs513[Pdat-1::GFP::SNN-1b, Pdat-1::mcherry, Podr-1::RFP] | this study | TV19305 |
| wyIs553[Pdat-1::UNC-10::3xGFP, Pdat-1::tdTomato::RAB-3, Podr-1] | this study | TV15119 |
| thoc-5(wy822); wyIs553[Pdat-1::UNC-10::3xGFP, Pdat-1::tdTomato::RAB-3, Podr-1] | this study | TV19407 |
| wyIs521[Pdat-1::CAT-1::GFP, Pdat-1::mcherry, Pord-1::RFP] | this study | TV114867 |
| thoc-5(wy822); wyIs521[Pdat-1::CAT-1::GFP, Pdat-1::mcherry, Pord-1::RFP] | this study | TV19726 |
| ztf-11(wy1077)[ztf-11::zf1::GFPnovo2]; zif-1(gk117) | this study | TV20812 |
| thoc-5(wy822); ztf-11(wy1077)[ztf-11::zf1::GFPnovo2] | this study | TV21659 |
| thoc-5(wy822); wyIs427[Pdat-1::GFP::ELKS-1, Pdat-1::mcherry, Podr-1::RFP]; wyEx6458[Pdat-1::THOC-5] | this study | TV16056 |
| thoc-2(ok961)/hTs[bli4(e937) let-?(q782) qIs48]; wyIs557[Pdat-1::GFP::ELKS-1, Pdat-1::tdTomato::RAB-3, Podr-1::GFP] | this study | TV16277 |
| thoc-1(wy817)/+; wyIs427[Pdat-1::GFP::ELKS-1, Pdat-1::mcherry, Podr-1::RFP] | this study | TV14850 |
| wyEx7354[Pdat-1::THOC-1::GFP]; thoc-1(wy817)/+; wyIs427[Pdat-1::GFP::ELKS-1, Pdat-1::mcherry, Podr-1::RFP] | this study | TV17802 |
| aly-1(ok1920) aly-2(wy924) aly-3(wy909); wyIs427[Pdat-1::GFP::ELKS-1, Pdat-1::mcherry, Podr-1::RFP] | this study | TV17992 |
| syd-2(wy1073)[GFP::syd-2] | this study | TV20871 |
| syd-2(wy1073)[GFP::syd-2]; thoc-5(wy822) | this study | TV20982 |
| rab-3(ox699)[GFP::FLP-on::rab-3]; Psnt-1::flp | this study | TV22398 |
| rab-3(ox699)[GFP::FLP-on::rab-3]; Psnt-1::flp; thoc-5(wy822) | this study | TV22397 |
| sng-1(ox706)[sng-1::GFP] | Jorgensen lab | EG9408 |
| sng-1(ox706)[sng-1::GFP]; thoc-5(wy822) | this study | TV21323 |
| rab-3(ox699)[GFP::FLP-on::rab-3]; wyEx8715[Pdat-1::flp; Podr-1::GFP] | this study | TV21223 |
| rab-3(ox699)[GFP::FLP-on::rab-3]; wyEx8715[Pdat-1::flp; Podr-1::GFP]; thoc-5(wy822) | this study | TV21801 |
| rab-3(ox699)[GFP::FLP-on::rab-3]; wyEx8715[Pdat-1::flp; Podr-1::GFP]; thoc-5(wy822) crh-1(tz2) | this study | TV21529 |
| rab-3(ox699)[GFP::FLP-on::rab-3]; wyEx8927[Ptph-1::flp; Podr-1::RFP] | this study | TV21944 |
| rab-3(ox699)[GFP::FLP-on::rab-3]; wyEx8927[Ptph-1::flp; Podr-1::RFP]; thoc-5(wy822) crh-1(tz2) | this study | TV22039 |
| rab-3(ox699)[GFP::FLP-on::rab-3]; wyEx8929[Punc-47::flp; Podr-1::RFP] | this study | TV21946 |
| rab-3(ox699)[GFP::FLP-on::rab-3]; wyEx8929[Punc-47::flp; Podr-1::RFP]; thoc-5(wy822) crh-1(tz2) | this study | TV22015 |
| rab-3(ox699)[GFP::FLP-on::rab-3]; wyEx8929[Punc-47::flp; Podr-1::RFP]; thoc-5(wy822) | this study | TV22020 |
| rab-3(ox699)[GFP::FLP-on::rab-3]; wyIs821[Pmig-13::flp; Podr-1::RFP]; thoc-5(wy822) | this study | TV22025 |
| rab-3(ox699)[GFP::FLP-on::rab-3]; wyIs821[Pmig-13::flp; Podr-1::RFP]; thoc-5(wy822) crh-1(tz2) | this study | TV22396 |
| rab-3(ox699)[GFP::FLP-on::rab-3]; wyIs821[Pmig-13::flp; Podr-1::RFP] | this study | TV22024 |
| cat-2(e1112) | CGC | CB1112 |
| thoc-2(ok961)/hTs[bli4(e937) let-?(q782) qIs48] | CGC | VC673 |
| wyIs700[Pdat-1::GFP; Podr-1::RFP] | this study | TV18678 |
| wyIs700[Pdat-1::GFP; Podr-1::RFP]; thoc-5(wy822) | this study | TV18846 |
| rab-3(ox699)[GFP::FLP-on::rab-3]; wyEx8715[Pdat-1::flp; Podr-1::GFP]; thoc-5(wy822); cat-2(e1112) | this study | TV22035 |
| rab-3(ox699)[GFP::FLP-on::rab-3]; wyEx8715[Pdat-1::flp; Podr-1::GFP]; dop-5(ok568) | this study | TV22046 |
| rab-3(ox699)[GFP::FLP-on::rab-3]; wyEx8715[Pdat-1::flp; Podr-1::GFP]; thoc-5(wy822); dop-5(ok568) | this study | TV22401 |
| rab-3(ox699)[GFP::FLP-on::rab-3]; wyEx8715[Pdat-1::flp; Podr-1::GFP]; pin-2(gk435511) | this study | TV22370 |
| rab-3(ox699)[GFP::FLP-on::rab-3]; wyEx8715[Pdat-1::flp; Podr-1::GFP]; pin-2(gk435511), chr-1(tz2) | this study | TV22371 |
| rab-3(ox699)[GFP::FLP-on::rab-3]; wyEx8715[Pdat-1::flp; Podr-1::GFP]; pin-2(gk435511); thoc-5(wy822) chr-1(tz2) | this study | TV22400 |
| wyIs427[Pdat-1::GFP::ELKS-1, Pdat-1::mcherry, Podr-1::RFP]; wyEx8629[Pdat-1::HisCL; Podr-1::GFP] | this study | TV21033 |
| wyIs427[Pdat-1::GFP::ELKS-1, Pdat-1::mcherry, Podr-1::RFP]; wyEx8712[Pdat-1::HisCL; Podr-1::GFP]; thoc-5(wy822) | this study | TV21209 |
| wyIs557[Pdat-1::GFP::ELKS-1, Pdat-1::tdTomato::RAB-3, Podr-1::GFP]; thoc-5(wy822); gsa-1(ce94) | this study | TV21136 |
| wyIs557[Pdat-1::GFP::ELKS-1, Pdat-1::tdTomato::RAB-3, Podr-1::GFP]; thoc-5(wy822); cmk-1(ok287) | this study | TV20576 |
| wyIs557[Pdat-1::GFP::ELKS-1, Pdat-1::tdTomato::RAB-3, Podr-1::GFP]; thoc-5(wy822); crtc-1(tm7084) | this study | TV20876 |
| wyIs557[Pdat-1::GFP::ELKS-1, Pdat-1::tdTomato::RAB-3, Podr-1::GFP]; thoc-5(wy822); pmk-1(km25) | this study | TV21330 |
| wyIs557[Pdat-1::GFP::ELKS-1, Pdat-1::tdTomato::RAB-3, Podr-1::GFP]; thoc-5(wy822); trp-4(sy695) | this study | TV21791 |
| wyIs557[Pdat-1::GFP::ELKS-1, Pdat-1::tdTomato::RAB-3, Podr-1::GFP]; thoc-5(wy822); dop-1(vs100) | this study | TV21660 |
| wyIs557[Pdat-1::GFP::ELKS-1, Pdat-1::tdTomato::RAB-3, Podr-1::GFP]; thoc-5(wy822); egl-30(ad805) | this study | TV21954 |
| wyIs557[Pdat-1::GFP::ELKS-1, Pdat-1::tdTomato::RAB-3, Podr-1::GFP]; thoc-5(wy822); kin-2(ce179) | this study | TV20140 |
| wyIs427[Pdat-1::GFP::ELKS-1, Pdat-1::mcherry, Podr-1::RFP]; thoc-5(wy822); dop-2(vs105); dop-3(vs106) | this study | TV21523 |
| wyIs427[Pdat-1::GFP::ELKS-1, Pdat-1::mcherry, Podr-1::RFP]; thoc-5(wy822); pkc-1(ok563) | this study | TV19788 |
| wyEx6497[Pdat-1::THOC-1::GFP; Podr-1::GFP] | this study | TV16143 |
| wyIs724[Pdat-1::THOC-2::GFP; Pdat-1::mcherry; Podr-1::RFP] | this study | TV19593 |
| wyIs737[Pdat-1::THOC-5::GFP, Pdat-1::mcherry; Podr-1::RFP] | this study | TV19785 |
| wyEx8628[Pdat-1::THOC-1::tdTomato; Pdat-1::crh-1a::GFP; Podr-1::RFP] | this study | TV21032 |
| wyEx7120[Pdat-1::aly-1::GFPnovo2; Pdat-1::mcherry, Podr-1::GFP] | this study | TV17347 |
| wyEx7122[Pdat-1::aly-2::GFPnovo2; Pdat-1::mcherry, Podr-1::GFP] | this study | TV17349 |
| wyEx7124[Pdat-1::aly-3::GFPnovo2; Pdat-1::mcherry, Podr-1::GFP] | this study | TV17351 |
| wyIs783[Prab-3::THOC-1::3xHA::tdTomato; Prab-3::crh-1a::GFP, Podr-1::RFP] | this study | TV21307 |
| wyEx8951[Prab-3::THOC-1::3xHA::tdTomato; Prgef-1:: GFP, Podr-1::RFP] | this study | TV21991 |
| wyEx8951[Prab-3::THOC-1::3xHA::tdTomato; Prgef-1:: GFP, Podr-1::RFP]; crh-1(tz2) | this study | TV22380 |
| thoc-1(wy817)/+; wyEx9278[Pdat-1::THOC-1(V338I)::GFP, Podr-1::GFP]; wyIs427[Pdat-1::GFP::ELKS-1, Pdat-1::mcherry, Podr-1::RFP] | this study | TV22654 |
| thoc-1(wy817)/+; wyEx9279[Pdat-1::THOC-1(V338I)::GFP, Podr-1::GFP]; wyIs427[Pdat-1::GFP::ELKS-1, Pdat-1::mcherry, Podr-1::RFP] | this study | TV22655 |
| thoc-1(wy817)/+; wyEx9338[Pdat-1::THOC-1(P1236T)::GFP, Podr-1::GFP]; wyIs427[Pdat-1::GFP::ELKS-1, Pdat-1::mcherry, Podr-1::RFP] | this study | TV22684 |
| thoc-1(wy817)/+; wyEx9337[Pdat-1::THOC-1(P1236T)::GFP, Podr-1::GFP]; wyIs427[Pdat-1::GFP::ELKS-1, Pdat-1::mcherry, Podr-1::RFP] | this study | TV22863 |
| thoc-1(wy817)/+; wyEx9353[Pdat-1::THOC-1(P1236T)::GFP, Podr-1::GFP]; wyIs427[Pdat-1::GFP::ELKS-1, Pdat-1::mcherry, Podr-1::RFP] | this study | TV22925 |
| wyEx9292[Pdat-1::CRE, Podr-1::GFP]; wyIs427[Pdat-1::GFP::ELKS-1, Pdat-1::mcherry, Podr-1::RFP]; thoc-5(wy822) crh-1(wy1152wy1154) | this study | TV22668 |
| wyEx9293[Pdat-1::CRE, Podr-1::GFP]; wyIs427[Pdat-1::GFP::ELKS-1, Pdat-1::mcherry, Podr-1::RFP]; thoc-5(wy822) crh-1(wy1152wy1154) | this study | TV22669 |
| rab-3(ox699)[GFP::FLP-on::rab-3]; wyEx8715[Pdat-1::flp; Podr-1::GFP]; thoc-5(wy822) crh-1(tz2); dop-5(ok568) | this study | TV23052 |
| rab-3(ox699)[GFP::FLP-on::rab-3]; wyEx8715[Pdat-1::flp; Podr-1::GFP]; thoc-5(wy822) crh-1(tz2); pin-2(gk435511) | this study | TV22400 |
| edIs6[Punc-119::GFP; rol-6(su1006)] | CGC | DP132 |
| edIs6[Punc-119::GFP; rol-6(su1006)]; thoc-5(wy822) | this study | TV19032 |
| lite-1(xu7); xuEx2165[Pdat-1::GCaMP6f+Pdat-1::sl2::mcherry] | this study | TQ6291 |
| lite-1(xu7); thoc-5(wy822); xuEx2165[Pdat-1::GCaMP6f+Pdat-1::sl2:mcherry] | this study | TQ6340 |
| wyEx6695[Pdat-1::rps-2::3xGFP, Pdat-1::mcherry, Podr-1::GFP] | this study | TV16704 |
| wyEx6695[Pdat-1::rps-2::3xGFP, Pdat-1::mcherry, Podr-1::GFP]; thoc-5(wy822) | this study | TV17593 |
| wyEx6697[Pdat-1::aman-2b::YFP, Pdat-1::mcherry, Podr-1::GFP] | this study | TV16706 |
| wyEx6697[Pdat-1::aman-2b::YFP, Pdat-1::mcherry, Podr-1::GFP]; thoc-5(wy822) | this study | TV17622 |
| wyEx6699[Pdat-1::rab-5::GFP, Pdat-1::mcherry, Podr-1::GFP] | this study | TV16708 |
| wyEx6699[Pdat-1::rab-5::GFP, Pdat-1::mcherry, Podr-1::GFP]; thoc-5(wy822) | this study | TV17594 |
| wyEx6702[Pdat-1::mito::GFP, Pdat-1::mcherry, Podr-1::GFP] | this study | TV16711 |
| wyEx6702[Pdat-1::mito::GFP, Pdat-1::mcherry, Podr-1::GFP]; thoc-5(wy822) | this study | TV17595 |
| wyEx6702[Pdat-1::SP-12::GFP, Pdat-1::mcherry, Podr-1::GFP] | this study | TV16863 |
| wyEx6702[Pdat-1::SP-12::GFP, Pdat-1::mcherry, Podr-1::GFP]; thoc-5(wy822) | this study | TV17592 |
| wyEx6781[Pdat-1::rab-6.2::GFP, Pdat-1::mcherry, Podr-1::GFP] | this study | TV16866 |
| wyEx6781[Pdat-1::rab-6.2::GFP, Pdat-1::mcherry, Podr-1::GFP]; thoc-5(wy822) | this study | TV17616 |
| wyEx6497[Pdat-1::THOC-1::GFP; Podr-1::GFP]; crh-1(tz2) | this study | TV23400 |
| wyIs724[Pdat-1::THOC-2::GFP; Pdat-1::mcherry; Podr-1::RFP]; crh-1(tz2) | this study | TV23437 |
| wyIs737[Pdat-1::THOC-5::GFP, Pdat-1::mcherry; Podr-1::RFP]; crh-1(tz2) | this study | TV23401 |
| wyEx7120[Pdat-1::aly-1::GFPnovo2; Pdat-1::mcherry, Podr-1::GFP]; crh-1(tz2) | this study | TV23402 |
| wyEx8581[Pdat-1::crh-1a::GFP, Podr-1::RFP] | this study | TV20899 |
| wyEx8581[Pdat-1::crh-1a::GFP, Podr-1::RFP]; thoc-5(wy822) | this study | TV20901 |
| rab-3(ox699)[GFP::FLP-on::rab-3]; wyEx8715[Pdat-1::flp; Podr-1::GFP]; thoc-5(wy822); pab-2(ok1851) | this study | TV22402 |
| rab-3(ox699)[GFP::FLP-on::rab-3]; wyEx8715[Pdat-1::flp; Podr-1::GFP]; pab-2(ok1851) | this study | TV22044 |
| wyIs700[Pdat-1::GFP; Podr-1::RFP]; pab-2(ok1851) | this study | TV21475 |
| wyIs700[Pdat-1::GFP; Podr-1::RFP]; thoc-5(wy822); pab-2(ok1851) | this study | TV21530 |
| wyIs700[Pdat-1::GFP; Podr-1::RFP]; crh-1(tz2) | this study | TV20417 |
| wyIs700[Pdat-1::GFP; Podr-1::RFP]; thoc-5(wy822) crh-1(tz2) | this study | TV20588 |
| wyIs427[Pdat-1::GFP::ELKS-1, Pdat-1::mcherry, Podr-1::RFP]; unc-120(st364) | this study | TV23430 |
| wyIs427[Pdat-1::GFP::ELKS-1, Pdat-1::mcherry, Podr-1::RFP]; unc-120(st364); thoc-5(wy822) | this study | TV23599 |
| Mouse strains | ||
| THOC5(fl/fl) | (Saran et al., 2013) | N/A |
| DAT-IRES-Cre (B6.SJL-Slc6a3tm1.1(cre)Bkmn/J) | JAX | Cat# JAX:006660; RRID:IMSR_JAX:006660 |
| Ai32 (B6;129S-Gt(ROSA)26Sortm32(CAG-COP4*H134R/EYFP)Hze/J) | JAX | Cat# JAX:012569; RRID:IMSR_JAX:012569 |
| ANTIBODIES | ||
| Rabbit anti SNB-1 | Nonet lab | Ab1092 |
| Rabbit anti SNT-1 | Nonet lab | Ab1095 |
| Rabbit anti UNC-64 | Nonet lab | Ab940 |
| Rabbit anti ELKS-1 | Nonet lab | Ab237 |
| Chicken anti SNG-1 | Nonet lab | Ab1165 |
| Rabbit anti-Chicken IgY (H+L) Secondary Antibody, HRP conjugate | Thermo Scientific | Cat# 31401 |
| Perox-IgG Fraction Monoc Mse Anti-Rab IgG, Lt Chn Spec (min X Bov,Gt,Arm Hms,Hrs,Hu,Ms,Rat,Shp Ig) | Jackson Immuno | Cat# 211–032-171 |
| GFP (goat) antibody biotin conjugated | Rockland inc. | Cat# 600–106-215; RRID:AB_218204 |
| Anti-Tyrosine Hydroxylase antibody | Abcam | Cat# ab112; RRID:AB_297840 |
| Anti-Dopamine Transporter antibody | Abcam | Cat# ab5990; RRID:AB_305226 |
| mono-clonal anti-HA antibody produced in mouse | Sigma | Cat# H3663; RRID:AB_262051 |
| Pierce™ Anti-HA Magnetic Beads | Thermo Scientific | Cat# 88836 |
| Mouse anti-actin, monoclonal (clone: C4) | MP Biomedicals | Cat# 8691001 |
| Monoclonal Anti-α-Tubulin antibody produced in mouse | Sigma | Cat# T6199; RRID:AB_477583 |
| Goat anti rabbit IgG H&L Alexa Fluor 488 | Abcam | Cat# ab150077; RRID:AB_2630356 |
| Goat anti rat IgG H&L Alexa Fluor 647 | Abcam | Cat# ab150167 |
| Mouse anti THOC1, monoclonal | Novus | Cat# NB100–174; RRID:AB_2202241 |
| Rabbit anti CREB | Sigma | Cat# PLA0205 |
| Oligonucleotides | ||
| Oligonucleotides for qPCR | this study | Table S2 |
| Software | ||
| ImageJ, Version 1.45k | NIH | https://imagej.nih.gov/ij/download.html |
| Metamorph, Version 7.7.9.0 | Molecular Devices | http://mdc.custhelp.com/app/answers/detail/a_id/15203/~/metamorph®-software-installation-files |
| Prism, Version 7 | GraphPad | https://www.graphpad.com/scientific-software/prism/ |
| Deposited Data | ||
| Raw and analyzed data | this study | https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE102300 |
| Critical commercial assay | ||
| ssofast evagreen supermix with low ROX | Biorad | Cat# 172–5211 |
| SuperScript® III First-Strand Synthesis SuperMix for qRT-PC | Invitrogen | Cat# 11752–050 |
| Chemicals | ||
| SuperSignal West Femto Maximum Sensitivity Substrate | Thermo Scientific | Cat# 34096 |
| NuPAGE® Novex® 4–12% Bis-Tris Protein Gels, 1.0 mm, 12 well | Life Technologies | Cat# NP0322BOX |
| NuPAGE® MOPS SDS Running Buffer (20X) | Life Technologies | Cat# NP0001 |
| NuPAGE® Transfer Buffer (20X) | Life Technologies | Cat# NP0006 |
| NuPAGE® LDS Sample Buffer (4X) | Life Technologies | Cat# NP0007 |
| Methanol | Sigma | Cat# 320390 |
| Blotting grade dry milk | Biorad | Cat# 170–6404 |
| Paraformaldehyde | Sigma | Cat# 158127 |
| Dextran Sulfate 50% Solution, 100 mL | Millipore | Cat# S4030 |
| E.coli tRNA | Roche | Cat# 10109541001 |
| UltraPure™ BSA (50 mg/mL) | Life Technologies | Cat# AM2618 |
| Nuclease-Free Water (not DEPC-Treated) | Life Technologies | Cat# AM9939 |
| 20X SSC | Life Technologies | Cat# AM9770 |
| DAPI (4’,6-Diamidino-2-Phenylindole, Dilactate) | Life Technologies | Cat# D3571 |
| Formamide (Deionized) | Life Technologies | Cat# AM9342 |
| Histamine dihydrochloride | Sigma | Cat# H7250 |
| Dopamine hydrochloride | Sigma | Cat# H8502 |
| RNasin Ribonuclease Inhibitor | Promega | Cat# N2115 |
| Complete mini EDTA-free protease inhibitor tablets | Roche | Cat# 11836170001 |
| TRIzol® LS Reagent | Life Technologies | Cat# 10296–010 |
| GlycoBlue™ Coprecipitant (15 mg/mL) | Thermofisher | Cat# AM9515 |
| Levamisol hydrochloride | Sigma | Cat# 31742 |
| RNAeasy Plus Micro Kit | Qiagen | Cat# 74034 |
| SMART-seq v4 Ultra low input RNA kit | Clontech | Cat# 634888 |
| Nextera XT DNA library preparation kit | Illumina | Cat# FC-131–1024 |
| Protease from Streptomyces griseus | Sigma | Cat# P6911 |
| Fetal Bovine Serum, certified, heat inactivated, US origin | Life Technologies | Cat# 10082–139 |
| Hoechst 33258, Pentahydrate (bis-Benzimide) - 10 mg⁄mL Solution in Water | Life Technologies | Cat# H3569 |
| Propidium Iodide - 1.0 mg⁄mL Solution in Water | Life Technologies | Cat# P3566 |
| Isofluorane | Vetone | Cat# 200–070 |
| Ketamine | Vedco | Cat# 50989–161-06 |
| xylazine | Lloyd | Cat# 139–236 |
| NBQX | Tocris | Cat# 1044 |
| (R)-CPP | Tocris | Cat# 0247 |
| SR95531 | Tocris | Cat# 1262 |
| Recombinant DNA | ||
| pCM362: pSM::Pdat-1::tdTomato-rab-3::unc10–3’UTR | this study | N/A |
| pCM367: pSM::Pdat-1::mcherry::unc10–3’UTR | this study | N/A |
| pCM377: pSM::Pdat-1::GFP::ELKS-1::unc-54 3’UTR | this study | N/A |
| pCM378: pSM::Pdat-1::GFP::snn-1::unc-54 3’UTR | this study | N/A |
| pCM413: pSM::Pdat-1::GFP::unc-54 3’UTR | this study | N/A |
| pCM429: pSM::Pdat-1::cat-1::GFP::unc-54 3’UTR | this study | N/A |
| pCM434: pSM::Pdat-1::unc-10::3xGFP::unc-54 3’UTR | this study | N/A |
| pCM483: pSM::Pdat-1::THOC-5::GFP::unc-54 3’UTR | this study | N/A |
| pCM485: pSM::Pdat-1::THOC-5::unc-54 3’UTR | this study | N/A |
| pCM493: pSM::Pdat-1::THOC-2::GFP::unc-54 3’UTR | this study | N/A |
| pCM496: pSM::Pdat-1::THOC-1::GFP::unc-54 3’UTR | this study | N/A |
| pCM526: pSM::Pdat-1::3xGFPnovo2::syd-2::unc-54 3’UTR | this study | N/A |
| pCM530: pSM::Pdat-1::aly-1::GFPnovo2::unc-54 3’UTR | this study | N/A |
| pCM531: pSM::Pdat-1::aly-2::GFPnovo2::unc-54 3’UTR | this study | N/A |
| pCM532: pSM::Pdat-1::aly-3::GFPnovo2::unc-54 3’UTR | this study | N/A |
| pCM562: pSM::Pdat-1::GFP::cla-1::unc-54 3’UTR | this study | N/A |
| pCM698: pSM::Pdat-1::CRH-1a::GFP::unc-54 3’UTR | this study | N/A |
| pCM708: pSM::Pdat-1::THOC-1::tdTomato::unc-54 3’UTR | this study | N/A |
| pCM710: pSM::Prab-3::CRH-1a::GFP::unc-54 3’UTR | this study | N/A |
| pCM711: pSM::Pdat-1::HisCL1::unc-54 3’UTR | this study | N/A |
| pCM712: pSM::Prab-3::THOC-1::3xHA::tdTomato::unc-54 3’UTR | this study | N/A |
| pCM763: pSM::Prgef-1:: GFP::let-858 3’UTR | this study | N/A |
| Other | ||
| EpiShear Probe Sonicator | Active Motif | Cat# 53051 |
| CFX96 Touch™ Real-Time PCR Detection System | Biorad | Cat# 1855196 |
| Zeiss Axio Observer Microscope with Yokagawa spinning disk head | Zeiss/Solamere Technology | N/A |
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