Figure 2.

CD34⁺ cellular viability is modestly reduced with MRI tracer. Results represent the paired analysis of repeated experiments assessing viability by Trypan blue, apoptosis by nonyl acridine orange (NAO) expression and yields after CD34⁺ cells were cultured with and without MRI tracer (10 mg/ml). Averages are indicated in heavy horizontal bars for viability (n = 10), NAO expression (n = 6), and yields (n = 7). Histograms of flow cytometric analysis of NAO staining in MRI tracer-labeled and unlabeled cells as well as unstained cells in shaded histogram. Cells undergoing apoptosis (NAO^low) are detected by a decrease in fluorescence (region defined by M1).