Table 3. Potential amino acid substitutions detected in the samples analysed in this study, compared to the reference Hic74 sequence [Hyriopsis cumingii].

Positions are derived from the sequence alignment shown in Figure 6. Dashes indicate that the position was not covered for that sample; question mark symbols indicate ambiguous substitutions. Hic74 coverages for each sample and supporting product ion spectra are presented in Figure 6—figure supplement 1, Figure 6—figure supplement 2, Figure 6—figure supplement 3, Figure 6—figure supplement 4, Figure 6—figure supplement 5, Figure 6—figure supplement 6, Figure 6—figure supplement 7, Figure 6—figure supplement 8, Figure 6—figure supplement 9, Figure 6—figure supplement 10, as well as in Figure 6—source data 1.

Hic74 [Hyriopsis cumingii]	A	A	A	A	A	A	A	G	D	G	S	E	G	A	A	L	V	G	L	I	A	G	A	Q	R	E
AA position	83	85	91	93	106	108	110	111	151	152	163	172	175	282	283	284	289	292	306	310	403	801	804	821	822	827
U. crassus	-	-	A	A	A	A	A	G	D	G	-	Q	G	A	?	L	?	G	L	F	A	-	-	Q	R	E
U. pictorum	-	-	?	?	?	-	-	-	-	-	-	Q	G	-	-	-	-	-	L	F	?	S	S	Q	R	E
M. margaritifera	-	-	-	A	-	-	-	-	-	-	-	Q	G	-	A	L	L	G	L	F	A	-	-	Q	R	E
P. auricularius	-	A	A	A	A	A	A	-	?	?	D	E	L	-	A	F	V	E	F	I	-	-	-	H	H	D
HavA	A	S	A	A	A	?	?	A	-	-	-	Q	G	A	A	L	L	G	L	F	-	-	-	Q	G	E
HavB	?	S	A	A	A	V	-	-	-	-	-	-	-	-	A	L	L	G	L	F	-	-	-	Q	G	E
HavC	?	S	A	A	A	V	?	A	D	G	G	-	G	?	A	L	L	G	L	F	-	G	S	Q	G	E