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. 2019 May 16;8:e43362. doi: 10.7554/eLife.43362

Figure 2. Evaluation of LISP2 expression in P. vivax infected huHep mice.

(A) Hepatic stages of P. vivax infected huHep mice were stained with DAPI (blue), a monoclonal antibody specific for P. vivax LISP2 protein (red) and a monoclonal antibody specific for P. vivax UIS4 protein (green). Representative images of LISP2-, LISP2+ and LISP2++ parasites at days 3, 5, 8 and 18 post-sporozoite infection are shown. (B) Day 8 LISP2+ trophozoite visualized for LISP2 (red) and UIS4 (green). The area in the yellow box is highlighted to show partial overlap of LISP2 and UIS4 localization at or near PVM prominence. Scale bar, 25 µm.

Figure 2.

Figure 2—figure supplement 1. UIS4 (up-regulated in infective sporozoites gene 4) protein localized at the PVM (Parasitophorous vacuole membrane) in P. cynomolgi liver stages.

Figure 2—figure supplement 1.

Liver stages infected with P. cynomolgi sporozoites were visualized at day 6 post-infection with anti-UIS4 polyclonal antibody and anti-HSP70 polyclonal antibody. White arrow indicates the characteristic UIS4 bright staining at one side of PVM, known as UIS4 prominence. UIS4 protein stains the parasite in a circumferential pattern surrounding the parasite in both hypnozoites and schizonts. Scale bar, 10 µm.