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. 2019 May 16;4(10):e124460. doi: 10.1172/jci.insight.124460

Figure 1. 4D light-sheet imaging of zebrafish embryos for assessment of myocardial contractility and valvular morphology.

Figure 1

(A) Schematic diagram and (B) photograph of the orthogonal optical pathway for single-sided illumination and dual-channel detection of the sample. (C) Outline of cardiac anatomy superimposed on a bright-field image from a zebrafish embryo at 5 days after fertilization (dpf) indicating orientation of the inflow tracts (IFTs) and OFTs (red arrows). (D) Schematic diagram of the embryonic heart (coronal section) highlighting the atrioventricular (AV) and ventriculobulbar (VB) valves, atrium, ventricle, OFT, and bulbus arteriosus. (E and F) Light-sheet fluorescence microscopy image of the embryonic heart at 5 dpf, showing (E) the open VB valve during systole and (F) the open AV valve during diastole. (G) 3D reconstruction of the embryonic heart during systole with VB and AV valves shown in red and orange, respectively.