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. 2019 May 16;4(10):e124213. doi: 10.1172/jci.insight.124213

Figure 1. Aged mice have remodeling of their bone marrow and expansion of MSC populations.

Figure 1

(A and B) Representative images of 2-photon intravital microscopy of calvaria; second harmonic generation (collagen; blue) and vasculature (70-kDa dextran; red). Scale bars: 100 μm. (C–E) Vascular (C), marrow (D), and collagen (E) volume (n = 5–7 mice per group). (F and G) Immunohistochemistry of osteocalcin (pink) and endomucin (brown). Scale bars: 10 μm. Black arrows indicate osteocalcin-positive osteoblasts. (H and I) Immunohistochemistry for LepR+ cells (brown). Scale bars: 100 μm. Black arrows indicate LepR+ perivascular cells. (J–R) Representative flow cytometry plots (J, M, O, and Q) and analysis quantification (K, L, N, P, and R) of marrow osteoblastic cells (OBCs: CD45–TER119–CD31–CD51+SCA1– cells, J and K), marrow multipotent stromal cell populations (MSCs: CD45–TER119–CD31–CD51+SCA1+ cells, J and L), PαS (CD45–TER119–CD31–CD140a+SCA1+ cells, M and N), PααV (CD45–TER119–CD31–CD140a+CD51+ cells, O and P) (n = 13–14 mice per group), and leptin receptor–positive (LepR+) (CD45–TER119–CD31–LepR+ cells, Q and R) (n = 9 mice per group). Each symbol represents an individual mouse; data represent mean ± SEM. P values, 2-tailed Student’s t test: **P < 0.01, ***P < 0.001.