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. 2019 May 16;4(10):e124213. doi: 10.1172/jci.insight.124213

Figure 2. Aged mice have expanded megakaryocyte-biased HSCs.

Figure 2

(A–G) Representative flow cytometry of CD41 expression and its quantification across LSK subsets in young and aged mice (n = 6 mice per group). (H) Principal component analysis (PCA) plot of single LT-HSCs from young and aged mice. Variance of each PCA is shown. (I) Violin plots of Selp (ANOVA P = 4.94 × 10–7), Vwf (P = 0.003051854), and Itgb3 (P = 6.38 × 10–5) expression from young and aged LT-HSCs (n = 76 young HSCs and 63 aged HSCs). (J and K) Quantification of CD61 (n = 9 mice per group) and of CD41/CD61 (n = 5 mice per group) expression across LSK subsets in young and aged mice. (L) Schematic representation of experimental design for analysis of megakaryocytic skewing potential of CD41+ LT-HSCs. (M and N) Donor platelet bias of sorted CD41+ (n = 5 recipients) versus CD41– (n = 4 recipients) LT-HSCs as percent donor and total number. (O) Quantification of in vitro megakaryocytic CFUs (CFU-MK) per 100 sorted CD41+ versus CD41– LT-HSCs (n = 4 per group). (G, J, K, and M–O). Each symbol represents an individual mouse; data represent mean ± SEM. P values, 2-tailed Student’s t test except as noted: *P < 0.05, **P < 0.01, ***P < 0.001.