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. 2019 May 16;4(10):e124213. doi: 10.1172/jci.insight.124213

Figure 3. Dysfunction in aged MSCs and regulation of HSCs by aged microenvironments.

Figure 3

(A) Schematic for in vitro coculture of HSCs and stromal cells. (B) Quantification of PαS cells from in vitro cultures derived from young and aged mice (n = 8 wells per group). (C) Quantification of fibroblastic CFUs (CFU-F) from young and aged mice (n = 5 young and aged mice). (D–F) Quantification of CD41 (D), CD61 (E), and CD41/CD61 (F) expression in LSKs grown on young or aged BMME cells (n = 12 young and aged per well). (G) Engraftment of HSCs grown on either young or aged BMME cells (n = 5 recipients per group). (B–F) Each symbol represents an individual mouse; data represent mean ± SEM. P values: (B–F) 2-tailed Student’s t test, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001; (G) P = 0.0014 results from a 2-way ANOVA between young and aged longitudinally, **P < 0.01 between young and aged longitudinally.