Figure 2. Cell selection and purification of Tregs.

(A) Enrichment of Tregs from peripheral blood G-CSF–mobilized progenitor cells, following depletion of CD34⁺ cells by immunomagnetic selection (left), selection of CD25⁺ by immunomagnetic selection (middle), and purification by FACS sorting of CD4⁺CD127^loCD25⁺ cells (right panel). Representative plots from 1 patient expressed as CD4⁺ and intracellularly stained FoxP3. (B) Suppression of the MLR by purified Tregs. The percentage of CFSE^dim populations of T cell–cultured HLA-mismatched PBMCs with/without Tregs (1:1 ratio) in allo-MLR, analyzed by FACS. Results are mean ± SD from 4 patients evaluated; P < 0.01, 2-tailed Student’s t test.