Figure 4. Immune reconstitution and TCR diversity of peripheral blood Tregs.

Reconstitution of naive Tregs as quantified by (A) CyTOF and (B) flow cytometric staining, both gated on CD4⁺CD25⁺CD127^lo. Patients on the modified protocol (n = 6) are compared with standard-of-care controls (n = 5) and initial protocol (n = 5) participants at 60 days after HCT. (C) TCRα and -β CDR3 repertoire diversity of FACS-purified CD4⁺CD25⁺CD127^lo Tregs at day 90 after HCT for patients on the modified protocol (n = 7) versus standard-of-care controls (n = 5). *P < 0.05 and **P < 0.01 respectively, Mann-Whitney U test, between control patients and patients on the modified protocol. Error bars represent mean ± SEM.