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. 2019 May 16;4(10):e125052. doi: 10.1172/jci.insight.125052

Figure 1. Schematic diagram of the self-complementary AAV (scAAV) backbone plasmid (pscw.CNP.Venus.Plp1miRNA) vector.

Figure 1

The scAAV backbone vector containing human CNP promoter, the coding sequence of fluorescent protein Venus, and the artificial miRNA sequence. The human CNP promoter was a 1.8-kilobase 5′ flanking sequence of the human CNP gene. The engineered miRNA cassette was placed in the 3′ UTR of the Venus sequence and consisted of a 5′ flanking region derived from miR155, a reverse complement of the 21-nucleotide target sequence, a 19-nucleotide loop sequence derived from miR155, nucleotides 1–8 and 11–21 of the sense target sequence, and a 3′ flanking region derived from miR155.