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. 2019 May 16;4(10):e122551. doi: 10.1172/jci.insight.122551

Figure 6. Self-reactive Abs exhibit long HCDR3s with frequent JH6 usage.

Figure 6

VHDJH rearrangements were recovered from transitional and mature B cells after single B cell culture. Based on the reactivity of the culture supernatant Abs, samples were grouped as follows: S-only-reactive (filled circles, n = 42); F+S-reactive (filled triangles, n = 49); F-only-reactive (open circles, n = 72); and unknown (open triangles, n = 67). Self-reactive Abs in this experiment represent Abs that reacted with RNP and/or Sm protein. (A) HCDR3 lengths are shown. Each symbol represents an individual sample. ***P < 0.001, NS: P > 0.05 determined by Mann-Whitney U test. (B) VH, D, and JH gene usages are shown. (C) The JH6 usage was compared between self-reactive (S-only-reactive plus F+S, n = 91) and non-self (F-only-reactive plus unknown, n = 139) groups. **P < 0.01 determined by χ2 test.