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. 2019 May 16;4(10):e127130. doi: 10.1172/jci.insight.127130

Figure 4. PHB knockdown promotes differentiation of neuroblastoma cells.

Figure 4

(A) Photomicrographs of Kelly cells after transduction with shRNAs against either PHB or GFP. Photomicrographs representative of 3 independent samples. (B) NTRK1 and NGFR mRNA expression measured by quantitative reverse transcription PCR (qRT-PCR) after PHB knockdown. Data represent mean ± SEM. n = 3, *P < 0.001, 2-tailed t test. (C) Heatmap showing significantly differentially expressed genes in IMR-5/75 after PHB knockdown. PHB, NTRK1, and NGFR are labeled in bold and denoted by arrowheads. n = 3, and P < 0.001. (D) GO analysis of differentially expressed genes after PHB knockdown. n = 3, Fisher’s exact test. (E) GSEA plot of neuronal differentiation after PHB knockdown. n = 3, Fisher’s exact test. Stated n values indicate number of biological replicates.