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. 2019 May 16;4(10):e127130. doi: 10.1172/jci.insight.127130

Figure 5. Ectopic expression of PHB promotes proliferation, migration, and dedifferentiation of neuroblastoma cells.

Figure 5

(A) Western blot analysis of cells after stable expression of PHB-V5 compared with empty vector control cells. Contrast of blots was enhanced equally for clarity. (B) Proliferation of IMR-5/75 cells stably expressing PHB-V5 compared with empty vector control cells as measured with the RTCA iCelligence system. Each condition was tested in triplicate. (C) Migration of IMR-5/75 and SH-SY5Y cells stably expressing PHB-V5 compared with empty vector control cells as measured by scratch assay. Data represent mean ± SD. n = 3, and **P < 0.05 for IMR-5/75; n = 1, and P > 0.05 for SH-SY5Y; 2-tailed t test. (D) Number of mounds formed by SH-SY5Y cells stably expressing PHB-V5 compared with empty vector control cells. Data represent mean ± SD. n = 3, and **P < 0.001; 2-tailed t test. (E) Expression of NTRK1 and NGFR measured by qRT-PCR. Data represent mean ± SD. ***P < 0.0003, and **P < 0.005; 2-tailed t test. Stated n values represent number of biological replicates.