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. 2019 May 16;4(10):e126533. doi: 10.1172/jci.insight.126533

Figure 3. IAV coinfection impairs Mtb-specific CD4+ T cell response.

Figure 3

C57BL/6 mice were infected via aerosol with a low dose of Mtb H37Rv and 12 days later coinfected i.n. with 1 × 104 PFU IAV (A/HH/05/09 H1N1). Lungs were analyzed by flow cytometry at indicated time points for (A) total CD4+ T cell numbers and frequencies among CD45+ cells, (B) ratio of CD4+ to CD8+ T cells (A and B, n = 4 mice per group, representative of 2 independent experiments), (C) for I-Ab ESAT-64–17–specific CD4+ T cells (day 18 n = 4 per group, 1 experiment; day 21 n = 4–5 per group, representative of 2 independent experiments), and (D and E) IFN-γ– and TNF-producing CD4+ T cells upon ex vivo restimulation with (D) ESAT-61–20 peptide or (E) PMA/Iono (n = 4 per group, day 15 and day 18 Mtb representative of 2 independent experiments, day 21 Mtb representative of 4 independent experiments). Each data point represents 1 mouse. *P ≤ 0.05; **P ≤ 0.01, ***P ≤ 0.001 determined by (A–E) 1-way ANOVA followed by Tukey’s multiple-comparison test.