Figure 8. Anti-IL10R treatment reduces Mtb load in coinfected mice.

C57BL/6 mice were infected via aerosol with a low dose of Mtb H37Rv and 12 days later coinfected with 1 × 10⁴ PFU IAV (A/HH/05/09 H1N1). (A) Timeline of experimental setup. Two hundred micrograms of anti-IL10R antibody (1B1.3) or isotype control (polyclonal rat serum IgG) was administered i.p. on day 5 after IAV infection (day 17 Mtb). (B) Lungs were collected at day 21 Mtb (day 9 IAV) and analyzed for bacterial burden (n = 7–8 per group, pooled data from 2 independent experiments). Each data point represents 1 mouse. **P ≤ 0.01, ***P ≤ 0.001 determined by (B) 2-way ANOVA followed by Tukey’s multiple-comparison test.