Table 2. Linear regression analysis showing associations of patient characteristics and maternal single nucleotide polymorphisms in drug disposition genes with log10 EFV plasma concentrations in maternal plasma, breast milk and infant plasma.
| Univariate linear regression | Multivariate linear regression | |||||
|---|---|---|---|---|---|---|
| Patient characteristic | βa (log10 EFV conc., 95% CI) | p value | Bonferroni p value |
Ba (log10 EFV conc., 95% CI) | p value | Bonferroni p value |
| Mother (n = 134) | ||||||
| Weight (kg) | ||||||
| Maternal plasma | -0.002 (-0.011, 0.008) | 0.73 | ||||
| Breast milk | -0.005 (-0.013, 0.004) | 0.30 | ||||
| Infant plasma | -0.004 (-0.013, 0.004) | 0.32 | ||||
| Maternal age (y) | ||||||
| Maternal plasma | -0.0003 (-0.012, 0.012) | 0.96 | ||||
| Breast milk | 0.006 (-0.007, 0.019) | 0.089 | ||||
| Infant plasma | -0.002 (-0.014, 0.01) | 0.71 | ||||
| Infant age (months) | ||||||
| Maternal plasma | 0.002 (-0.016, 0.02) | 0.80 | ||||
| Breast milk | 0.003 (-0.021, 0.027) | 0.80 | ||||
| Infant plasma | -0.026 (-0.043, -0.009) | 0.004 | 0.064 | -0.032 (-0.047, -0.016) | 9.9 x 10-5 | 0.0016 |
| Time post-dose (h) | ||||||
| Maternal plasma | 0.002 (-0.01, 0.014) | 0.78 | ||||
| Breast milk | 0.011 (-0.006, 0.027) | 0.20 | ||||
| Infant plasma | 0.016 (-0.005, 0.028) | 0.006 | 0.096 | 0.017 (0.007, 0.027) | 0.001 | 0.016 |
|
CYP2B6
516G>T (rs3745274) |
||||||
| Maternal plasma | 0.24 (0.17, 0.31) | 1.9 x 10-9 | 3.0 x 10-8 | 0.24 (0.17, 0.31) | 1.9 x 10-9 | 3.0 x 10-8 |
| Breast milk | 0.18 (0.098, 0.27) | 4.7 x 10-5 | 7.5 x 10-4 | 0.18 (0.098, 0.27) | 4.7 x 10-5 | 7.5 x 10-4 |
| Infant plasma | 0.18 (0.10, 0.26) | 2.3 x 10-5 | 3.7 x 10-4 | 0.19 (0.11, 0.27) | 6.0 x 10-6 | 3.7 x 10-4 |
|
CYP2B6
983T>C (rs28399499) |
||||||
| Maternal plasma | 0.061 (-0.098, 0.22) | 0.45 | ||||
| Breast milk | 0.043 (-0.13, 0.22) | 0.63 | ||||
| Infant plasma | -0.051 (-0.21, 0.11) | 0.53 | ||||
|
CYP2B6
c.485-18C>T (rs4803419) |
||||||
| Maternal plasma | -1.3E-5 (-0.17, 0.17) | 1.00 | ||||
| Breast milk | 0.008 (-0.18, 0.2) | 0.93 | ||||
| Infant plasma | -0.038 (-0.21, 0.14) | 0.67 | ||||
|
NR1I3
c.540C>T (rs2307424) |
||||||
| Maternal plasma | -0.091 (-0.24, -0.061) | 0.24 | ||||
| Breast milk | 0.025 (-0.14, 0.19) | 0.76 | ||||
| Infant plasma | -0.044 (-0.20, 0.11) | 0.57 | ||||
|
NR1I3
c.152-1089T>C (rs3003596) |
||||||
| Maternal plasma | -0.009 (-0.11, 0.087) | 0.85 | ||||
| Breast milk | -0.020 (-0.12, 0.08) | 0.69 | ||||
| Infant plasma | 0.042 (-0.055, 0.14) | 0.39 | ||||
|
ABCB1
4046A>G (rs1045642) |
||||||
| Maternal plasma | 0.12 (-0.026, 0.27) | 0.11 | ||||
| Breast milk | -0.011 (-0.17, 0.15) | 0.89 | ||||
| Infant plasma | -0.095 (-0.24, 0.053) | 0.21 | ||||
|
ABCB1
4036A>G (rs3842) |
||||||
| Maternal plasma | 0.037 (-0.1, 0.18) | 0.59 | ||||
| Breast milk | 0.071 (-0.075, 0.22) | 0.34 | ||||
| Infant plasma | 0.084 (-0.054, 0.22) | 0.23 | ||||
|
ABCB1
1236C>T (rs1128503) |
||||||
| Maternal plasma | 0.067 (-0.066, 0.2) | 0.32 | ||||
| Breast milk | -0.030 (-0.18, 0.12) | 0.69 | ||||
| Infant plasma | -0.052 (-0.19, 0.082) | 0.44 | ||||
|
CYP2A6
48T>G (rs28399433) |
||||||
| Maternal plasma | 0.058 (-0.22, 0.34) | 0.68 | ||||
| Breast milk | 0.11 (-0.40, 0.18) | 0.44 | ||||
| Infant plasma | 0.019 (-0.26, 0.30) | 0.89 | ||||
|
ABCB5
c.2908G>A (rs6461515) |
||||||
| Maternal plasma | 0.043 (-0.053, 0.14) | 0.38 | ||||
| Breast milk | 0.070 (-0.034, 0.18) | 0.18 | ||||
| Infant plasma | -0.039 (-0.14, 0.057) | 0.42 | ||||
|
ABCG2
c.1728-46G>A (rs2231164) |
||||||
| Maternal plasma | -0.020 (-0.15, 0.11) | 0.76 | ||||
| Breast milk | 0.11 (-0.050, 0.27) | 0.18 | ||||
| Infant plasma | 0.010 (-0.12, 0.14) | 0.88 | ||||
|
ABCG2
78551A>G (rs2622604) |
||||||
| Maternal plasma | -0.029 (-0.19, 0.13) | 0.72 | ||||
| Breast milk | -0.065 (-0.24, 0.11) | 0.47 | ||||
| Infant plasma | -0.11 (-0.27, 0.047) | 0.16 | ||||
a
β is regression coefficient, which represents incremental change in log10 EFV concentration per unit change in a patient characteristic.