Fig. 2.

Effect of SPARC on omental adipocyte-induced OvCa cell proliferation. a Schema of the effect of human and murine omental adipocytes on GFP-OvCa cells proliferation. b Line graphs representing means ± SEM of changes in the proliferation (GFP fluorescence) of ID8-GFP cells over-layed on top of SP^+/+ and SP^–/– adipocytes in the presence or absence of 5 µg/ml SPARC, over 72 h. c Means ± SEM of changes of tumours volumes after ID8 SC injection either alone or with SP^+/+ and SP^–/– adipocytes (1:2) in athymic nude mice. *p < 0.05, between experimental conditions starting at week 3 post-injection (n = 8/group; two-way ANOVA with Tukey’s multiple comparison test). d Means ± SEM of changes in the proliferation (GFP fluorescence) of GFP-labeled human OvCa cells on top of genetically engineered human adipocytes over 72 h. (n = 6/experimental condition). *P < 0.05 comparing hAdi-SP and hAdi-shSP with their corresponding vector control at the indicated time points, two-way ANOVA. e Changes in the proliferation (GFP fluorescence) of OvCa cells over-layed on top of adipocytes in presence or absence of 5 µg/ml SPARC over 72h. (n = 6/experimental condition). *p < 0.05, **P < 0.001, ***p < 0.0001, comparing proliferation of GFP-cells on uncoated UC, wells (black), matrigel (blue) and adipocytes (red) in presence and absence of SPARC, two-way ANOVA with Tuckey's multiple comparison test