Fig. 3.

Adipocyte-SPARC suppresses the reciprocal transcriptional activity of pro-inflammatory/adipogenic factors. a Schematic illustration of the experimental design and the trans-well assays for the single and co-cultures. b The levels of adipokines in SP^+/+ and SP^–/– omental adipocytes and ID8 cells in single and in co-cultures for 24 h was determined by qRT-PCR. c Transcriptional activity of cEBP, NFκB, and AP-1 in primary SP^+/+ and SP^–/– omental adipocytes and ID8 cells in single and in co-cultures was determined by measuring the luciferase reporter activity in each cell type. Results were normalized to fold change of DNA content of each cell type measured before and after the experiment as determined by CyQuant assay. Bars represent mean ± SEM from one of three experiments, performed in triplicate. *p < 0.05 Student’s t-test comparing SP^+/+ and SP^–/– adipocytes; ^#p < 0.05 Student’s t-test comparing adipocytes in single to co-cultures with ID8 cells; and **p < 0.05 Student’s t-test comparing ID8 cells in single to co-culture with SP^+/+ and SP^–/– adipocytes