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. 2019 May 31;39(5):BSR20181457. doi: 10.1042/BSR20181457

Figure 3. DUXAP10 si-RNA inhibited HCC metastasis and epithelial–mesenchymal transition.

Figure 3

Inhibition of DUXAP10 attenuates the cell migration, invasion, and EMT process (A,B). Transwell assays were used to determine cell migration and invasion status of HCC cells. Cells were plated in the top chamber of the inserts and cultured in the serum-free DMEM. Total 500 μl of 10% serum-containing DMEM were added in the lower chamber of the well. For migration test, the cells were allowed to migrate for 24 h and then removed from the upper chamber. Cells were seeded on Matrigel-coated membrane inserts for invasion experiment. Cells appeared in the underside of the membrane were stained with crystal violet and counted (**P<0.01 Hep G2 vs si-DUXAP10-Hep G2; ##P<0.01 SMMC7721 vs si-DUXAP10-SMMC7721). (C) EMT-related factors, the E-cadherin, N-cadherin, and vimentin, were determined by western blotting. (D) Ratio of E-cadherin to GAPDH has been calculated (**P<0.01 Hep G2 vs si-DUXAP10-Hep G2; ##P<0.01 SMMC7721 vs si-DUXAP10-SMMC7721). (E) Ratio of N-cadherin to GAPDH has been calculated (**P<0.01 Hep G2 vs si-DUXAP10-Hep G2; #P<0.05 SMMC7721 vs si-DUXAP10-SMMC7721). (F) Ratio of N-cadherin to GAPDH has been calculated (**P<0.01 Hep G2 vs si-DUXAP10-Hep G2; ##P<0.01 SMMC7721 vs si-DUXAP10-SMMC7721).