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. 2019 May 26;12(8):1015–1025. doi: 10.1016/j.tranon.2019.05.003

Figure 3.

Figure 3

Suppressing SRC signaling enhances JAKi-induced apoptosis in human ovarian cancer cells. SKOV3 (A & B) and MDAH2774 (C & D) cells were treated with JAKi and SRCi, either alone or in combination, for 48 hours. Apoptosis was determined by flow cytometry using Annexin V and PI staining (A & C) or by Western blot for cleaved poly-ADP ribose polymerase (PARP) and cleaved caspase-3 by (B & D). *, P < .05, **, P < .005; ***, P < .0005, combination vs. JAKi alone or SRCi alone. (E) SKOV3 cells were transfected with siRNA against SRC or control siRNA and treated with JAKi (5 μM) or (F) transfected with siRNA against JAK1 or STAT3 and treated with SRCi (saracatinib,10 μM). After 48 hours, cells were harvested and evaluated for apoptosis using annexin V staining. *, P < .05; **, P < .005, vs. control siRNA.