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. 2019 May 30;9:8069. doi: 10.1038/s41598-019-44559-8

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Speed of Endocytotic ZIKV-FP-Laden Vesicles Decreases the Most in Astrocytes. In astrocytes, the average speed of ZIKV-BR- and ZIKV-UG-laden vesicles significantly increased with longer hpi, whereas the speed of vesicles laden with ZIKV-FP significantly decreased. In neurons, the average vesicle speed exhibited the most prominent increase at 36 hpi, regardless of the virus strain, and then declined the most in ZIKV-FP-laden vesicles. In neuroblastoma SK-N-SH cells, the average speed of vesicles increased until 36 hpi, whereas in Vero E6 cells, the speed of virus-laden vesicles either decreased or remained unchanged. The number of analyzed cells is the same as in Figs 4, S2 and S3. Data were collected from one experiment, performed in duplicates and are represented as means ± SEM (One Way ANOVA, *p < 0.05). The average speed was calculated for the same vesicles as shown in Fig. 4.