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. 2019 May 30;9:8069. doi: 10.1038/s41598-019-44559-8

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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ZIKV Infection of Mosquito C6/36 Cells Does Not Affect the Mobility of DiD-ZIKV-laden vesicles. (a) Mosquito C6/36 cells infected with DiD-labeled ZIKV-BR at 12, 36, and 84 hpi. DIC images (left panels), fluorescent micrographs depicting punctate signals of DiD-labeled ZIKV-BR-laden vesicles (middle panels), fluorescent micrographs depicting DiD-labeled ZIKV-BR-laden vesicles with superimposed vesicle trajectories (right panels). Scale bars, 10 µm. Circles denote the starting positions of the vesicle trajectories (white lines). (b) Graphs represent the TL and MD of vesicles in C6/36 cells infected with strains ZIKV-BR, ZIKV-FP, and ZIKV-UG. The TL and MD of vesicles remained similar at all hpi; ZIKV-BR, p = 0.081 (TL), p = 0.532 (MD); ZIKV-FP, p = 0.767 (TL), p = 0.838 (MD); ZIKV-UG, p = 0.063 (TL), p = 0.465 (MD). The number of vesicles analyzed is denoted on the graphs. 5–9 cells per time period and per strain were analyzed. Data were collected from one experiment done in duplicates and are represented as means ± SEM (One Way ANOVA, *p < 0.05).