Fig. 2.

NADP(H) cleavage characterization. a Time course cleavage of pure NADPH (1 mM) and NADP⁺ (1 mM) by purified human full-length NOCT (0.5 μM) at 22 °C, monitored by ion-exchange chromatography (IEC). b 60-min NADPH incubation with the catalytic domains of PDE12, CNOT6L, and NOCT as in a. c Measurement of kinetic parameters for the catalytic domain of human NOCT (4.8 μM). d Western blot analysis of full-length human FLAG-NOCT (C-terminal tag) expressed in A549 cells for 18 h. e NADPH (1 mM) cleavage by FLAG-NOCT purified from human cells. f Nicotinamide metabolite composition in WT and NOCT^−/− cells (Supplementary Data 2). Six replicates from three WT samples are compared against four replicates from two independently generated NOCT^−/− clones. Error bars are standard error (S.E.). Source data are provided as a Source Data file