Fig. 7.

TM hairpins of FAM134B are required for ER fragmentation. a Immunofluorescence of HA and endogenous calnexin in U2OS cells transiently overexpressing HA-tagged FAM134B (left to right): wild-type (WT), LIR mutant (LIR mut), single hairpin deletions (ΔTM12 and ΔTM34), and the double hairpin deletion (ΔTM12 + TM34). Scale bars 10 μm. b Quantification of U2OS cells with fragmented ER (≥5 ER fragments per cell) after transient over-expression of wild-type and mutant forms of FAM134B. Error bars indicate s.d. from triplicates (red filled circles) and *Denotes p < 0.05 in two-sample Student’s t-tests. c Immunofluorescence of endogenous calnexin in un-transfected U2OS cells. Scale bar 10 μm