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. 2019 May 30;10:2366. doi: 10.1038/s41467-019-10272-3

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — Structure of the connector. a Left: a wire-frame structural model of a gp10 monomer shown in the prohead connector density. The density map of the prohead connector is drawn at a contouring level of 3.4 σ (magenta); right: zoom-ins showing the extended distal end of gp10 N-terminus at three different contouring levels. b Structure superposition of a prohead connector monomer (red) and a crystal structure connector monomer (black). The vertical black line indicates the position of the symmetry axis. c Left: ribbon diagrams showing the interactions between the prohead capsid and the gp10 N termini of a randomly placed prohead connector in a fivefold reconstructed prohead connector envelope. The connector is colored red. The N termini of the gp10 monomers are marked with blue balls. Yellow stars indicate gp10 monomers that have steric clashes with the capsid. The HK97-P subdomains are colored green. The E loops are colored magenta; right: plots of the clashscores (blue solid line) and the minimum distances (red dash line) against the rotational positions of the connector. The connector positions were generated by rotating the connector around its 12-fold symmetry axis with a step size of 1 degree. The clashscore calculated using the program phenix.molprobity is defined as 1000 × (number of bad overlaps)/(number of atoms). The minimum distance at each position was calculated using 12 Tyr122 Cα atoms of the connector and 5 Trp156 Cα atoms of the capsid. d Structure superposition of the prohead connector (red) and the mature head connector (blue) showing conformational changes of the connector upon head maturation. Top: top view; middle: side view; bottom: a schematic diagram showing possible level motion of the gp10 monomers. e Ribbon and shadowed-surface diagrams showing the contact sites of the connector to the mature capsid. The contacts are highlighted with black lines. Top: ribbon and shadowed-surface; bottom: electrostatic surface. Negative and positive electrostatic potentials are colored red and blue, respectively. The scale bar represents 5 nm