Fig. 4.

Adipose-specific HuR ablation suppresses adipocyte lipolysis. a EpiWAT explants were prepared from HFD-fed control and HuR^AKO mice, and stimulated with 1 μM ISO. FFA and glycerol release were measured (normalized to total protein levels). FFA release rates were calculated (n = 6), *comparison of HuR^AKO vs. control. b HFD-fed control and HuR^AKO mice were injected with CL316243 (1 mg kg⁻¹ body weight). Serum FFA and glycerol levels were measured (n = 6), *comparison of HuR^AKO vs. control. c SVFs were isolated from control and HuR^AKO mice, differentiated to adipocytes, and stimulated with 10 μM ISO. Glycerol and FFA release were calculated (normalized to total protein levels) (n = 4), *comparison of HuR^AKO vs. control. d Differentiated 3T3-L1 adipocytes were infected with adenovirus expressing GFP or HuR for 48 h. Cell extracts were immunoblotted with antibodies against HuR and β-actin. Adipocytes were stimulated with 10 μM ISO, and FFA and glycerol release were calculated (normalized to total protein levels) (n = 4), *comparison of HuR vs. GFP. Data are represented as mean ± SEM. Significance was determined by Student’s t test analysis, *P < 0.05. Source data are provided as a Source Data file