Fig. 5.

HuR regulates ATGL mRNA stability. a Western blot analysis of HuR and ATGL expression in adipose tissue (epiWAT and ingWAT) of 20-week-old C57BL/6J and ob/ob mice and quantification (n = 3), *comparison of ob/ob vs. C57. b Eight-week-old male C57BL/6J mice were fed with an HFD for an additional 12 weeks; western blot analysis of HuR and ATGL in adipose tissue and quantification (n = 3), *comparison of HFD (12w) vs. HFD (0w). c SVFs were isolated from control and HuR^AKO mice and differentiated to adipocytes. Western blot analysis of ATGL and quantification (n = 3), *comparison of HuR^AKO vs. control. d Differentiated 3T3-L1 adipocytes were infected with adenovirus expressing GFP or HuR for 48 h. Western blot analysis of ATGL protein level and quantification (n = 3), *comparison of HuR vs. GFP. e 3T3-L1 adipocytes were stimulated with CMLD-2 (30 μM) for 24 h. Western blot analysis of ATGL protein level and quantification (n = 3), *comparison of CMLD-2 vs. DMSO. f Differentiated 3T3-L1 adipocytes were stimulated with CMLD-2 (30 μM) or DMSO for 24 h. RNA immunoprecipitation with anti-HuR antibody or control IgG. g SVFs isolated from control and HuR^AKO mice were differentiated to adipocytes and stimulated with 10 µg mL⁻¹ actinomycin D. ATGL mRNA level was determined by qPCR (n = 4), *comparison of HuR^AKO vs. control. Data are represented as mean ± SEM. Significance was determined by Student’s t test analysis, *P < 0.05. Source data are provided as a Source Data file