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. 2019 May 24;10:1032. doi: 10.3389/fmicb.2019.01032

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Copyright © 2019 Goltermann, Yavari, Zhang, Ghosal and Nielsen.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Peptide-PNA uptake monitored by flow cytometry. E. coli culture incubated with BODIPY fluorophore labeled peptide-PNA with different PNA length was profiled by flow cytometry. (A) Full profiles of E. coli cultures incubated with each of the three peptide-PNA conjugates or without PNA. Background fluorescence extends to approx. 130 fluorescence units. (B) Excerpt from (A, box) showing only the part of the bacterial population containing the labeled peptide-PNA-conjugate. (C) Percentage of bacterial cells with peptide-PNA uptake.