Table 1.

Peptide-PNA-conjugates used (mm = mismatch).

PNA	Peptide	PNA sequence	Length	Target
2301	–	CTC ATA CTC T	10	acpP
4223	–	TG CTC ATA CTC T	12	acpP
4224	–	A GTG CTC ATA CTC T	14	acpP
4226	–	CG ATA GTG CTC ATA CTC T	18	acpP
3961	–	CTC TTA CAC T	10	mm for PNA2301
4521	–	TG CTC TTA CAC T	12	mm for PNA4223
4522	–	A GTG CTC TTA CAC T	14	mm for PNA4224
4524	–	CG ATA GTG CTC TTA CAC T	18	mm for PNA4226
4720	H-(KFF)3K-eg1-	CACATACTCT	10	mm for PNA2108
4721	H-(KFF)3K-eg1-	CTCTTACTCT	10	mm for PNA2108
4722	H-(KFF)3K-eg1-	CTCATAGTCT	10	mm for PNA2108
4723	H-(KFF)3K-eg1-	CTCATACTGT	10	mm for PNA2108
5077	H-(KFF)3K-eg1-	TA CTC T	6	acpP
5079	H-(KFF)3K-eg1-	C ATA CTC T	8	acpP
5080	H-(KFF)3K-eg1-	TC ATA CTC T	9	acpP
2108	H-(KFF)3K-eg1-	CTC ATA CTC T	10	acpP
5082	H-(KFF)3K-eg1-	G CTC ATA CTC T	11	acpP
5083	H-(KFF)3K-eg1-	TG CTC ATA CTC T	12	acpP
5164	H-(KFF)3K-eg1-	A GTG CTC ATA CTC T	14	acpP
5166	H-(KFF)3K-eg1-	ATA GTG CTC ATA CTC T	16	acpP
5168	H-(KFF)3K-eg1-	CG ATA GTG CTC ATA CTC T	18	acpP
5396	H-(KFF)3K-eg1-	TAC ACT	6	mm for PNA5077
5270	H-(KFF)3K-eg1-	CT TAC ACT	8	mm for PNA5079
5271	H-(KFF)3K-eg1-	TCT TAC ACT	9	mm for PNA5080
5272	H-(KFF)3K-eg1-	C TCT TAC ACT	10	mm for PNA2108
5273	H-(KFF)3K-eg1-	GC TCT TAC ACT	11	mm for PNA5082
5274	H-(KFF)3K-eg1-	TGC TCT TAC ACT	12	mm for PNA5083
5404	H-(KFF)3K-eg1-	AG TGC TCT TAC ACT	14	mm for PNA5164
5406	H-(KFF)3K-eg1-	A TAG TGC TCT TAC ACT	16	mm for PNA5166
5408	H-(KFF)3K-eg1-	CGA TAG TGC TCT TAC ACT	18	mm for PNA5168
3986	H-(R-Ahx)6-(β-Ala)-	CTC ATA CTC T	10	acpP
4227	H-(R-Ahx)6-(β-Ala)-	TG CTC ATA CTC T	12	acpP
4228	H-(R-Ahx)6-(β-Ala)-	A GTG CTC ATA CTC T	14	acpP
4229	H-(R-Ahx)6-(β-Ala)-	ATA GTG CTC ATA CTC T	16	acpP
4230	H-(R-Ahx)6-(β-Ala)-	CG ATA GTG CTC ATA CTC T	18	acpP
3987	H-(R-Ahx)6-(β-Ala)-	C TCT TAC ACT	10	mm for PNA3986
4500	H-(R-Ahx)6-(β-Ala)-	TGC TCT TAC ACT	12	mm for PNA4227
4501	H-(R-Ahx)6-(β-Ala)-	AG TGC TCT TAC ACT	14	mm for PNA4228
4502	H-(R-Ahx)6-(β-Ala)-	A TAG TGC TCT TAC ACT	16	mm for PNA4229
4503	H-(R-Ahx)6-(β-Ala)-	CGA TAG TGC TCT TAC ACT	18	mm for PNA4230
4099	H-(R-Ahx-R)4-Ahx-(β-Ala)-	CTC ATA CTC T	10	acpP
4246	H-(R-Ahx-R)4-Ahx-(β-Ala)-	TG CTC ATA CTC T	12	acpP
4247	H-(R-Ahx-R)4-Ahx-(β-Ala)-	A GTG CTC ATA CTC T	14	acpP
4248	H-(R-Ahx-R)4-Ahx-(β-Ala)-	ATA GTG CTC ATA CTC T	16	acpP
4288	H-(R-Ahx-R)4-Ahx-(β-Ala)-	CG ATA GTG CTC ATA CTC T	18	acpP
5631	H-(KFF)3K-eg1-Cys(BODIPY)-	TA CTC T	6	acpP
5491	H-(KFF)3K-Cys(BODIPY)-	CTC ATA CTC T	10	acpP
5629	H-(KFF)3K-eg1-Cys(BODIPY)-	A GTG CTC ATA CTC T	14	acpP

The PNAs were synthesized as previously reported (Good et al., 2001; Ghosal et al., 2013). eg1, 8-amino-3,6-dioxaoctanoic acid; Ahx, 6-aminohexanoic acid; Cys, cysteine; BODIPY, 4,4-difluoro-4-bora-3a,4a-diaza-s-indacene. Underlined: 10 nucleobase common motif. Bold and underlined: mismatched nucleobases.