Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 May 24;12:133. doi: 10.3389/fnmol.2019.00133

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2019 Vaithia, Vullo, Peng, Alijevic and Kellenberger.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

Conserved ΔF signal patterns in voltage-clamp fluorometry (VCF) experiments. All experiments of this figure are from hASIC1a expressed in Xenopus oocytes, voltage-clamped to −40 mV. (A–C) Different views of the structural model of hASIC1a described in legend of Figure 7, showing in (B) a close-up of the acidic pocket, in (C) a close-up of the wrist, indicating the positions of the residues that were mutated to Cys to attach the fluorophore. (D) Representative current (black) and fluorescence (red) traces of Cys and Cys-Trp mutants in the hASIC1a-D212 background. (E) Traces of the corresponding Cys and Cys-Trp mutants in the background of hASIC1a-G212. (D,E) Fluorophore-labeled oocytes were exposed to the stimulation pH6.0 from a conditioning pH7.4. Black arrows point to fast ΔF components. (F,G) Scatter dot plots comparing the fluorescence rise time (red) with the current rise time (F) or decay time (G) in response to an acidic stimulation at pH6.0 from a conditioning pH7.4, n = 4–9.