Figure 3.

Exogenous L-lactate does not affect the number of DCX⁺BrdU⁺ cells in the dentate gyrus. (A) Experiment design: Mice were i.p. injected with 1.75 g/kg L-lactate solution (n = 4) or PBS (n = 4) and were then injected with BrdU for 2 days at 8 h intervals and sacrificed 5 days after the last injection. (B) Total number of DCX⁺/BrdU⁺ cells in the DG; DCX⁺/BrdU⁺ cells were counted for each experimental animal’s DG and total DG population estimation was extrapolated. Results were averaged across treatment group. No significant differences were found between L-lactate and PBS throughout the DG (P > 0.05). (C) DCX⁺/BrdU⁺ averaged cell distribution according to the distance from Bregma position. DCX⁺/BrdU⁺ cell distribution was significantly higher in L-lactate group compared with PBS treatment (^∗p < 0.01).