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. 2018 Dec 21;9(3):516–525. doi: 10.1016/j.apsb.2018.12.006

Figure 2.

Fig. 2

Effect of SAD on cell cycle and apoptosis. (A) The cell cycle analysis was determined by PI staining and flow cytometry cell quest software. S1 and S1-MI-80 cells were treated with 4 μmol/L SAD for 12, 24, 48, and 72 h, respectively. The content of G2/M phase was increased in a time-dependent pattern. (B) Histograms of cell cycle distribution in non-treated and treated S1 and S1-MI-80 cells. (C) S1 and S1-MI-80 cells were treated with SAD (4 μmol/L) for four different time points. Western blot analysis was used to detect the levels of CDC2, p-CDC2 and cyclin B1 protein after SAD treatment. (D) SAD-mediated cell apoptosis in S1 and S1-MI-80 cells were detected by flow cytometer. (E) Cells were incubated for 0, 24, 48 and 72 h in the presence or absence of SAD. The induction of cell apoptosis was detected by flow cytometry. *P < 0.05, **P < 0.01 and ***P < 0.001 vs. the control. Data were presented as mean±SD from triplicate experiments.